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In the year 1963, the two enzymes respon...

In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in E.coli were isolated. One of these added methyl groups to host DNA, while the other cut DNA. The latter was called

A

restriction endonuclease

B

restriction exonuclease

C

Type III restriction enzyme

D

plasmid

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The correct Answer is:
A
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A plasmid has two antibiotic-resistance genes, one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the ones that

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AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. In the year 1963, the two enzymes responsible for restricting the grow...

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  2. DNA fragments are:

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  3. The process of separation and purification of expressed protein before...

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  4. The DNA fragments separated on an agarose gel can be visualised after ...

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  5. Stirred tank bioreactors have been designed for

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  6. A foreign DNA and plasmid cut by the same restriction endonuclease ca...

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  7. Which of the following is not a component of downstream processing?

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  8. Which of the following restriction enzymes produces blunt ends?

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  9. The taq polymerase enzyme is obtained from:

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  10. Which of the following is not required for any of the techniques of DN...

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  11. Which of the following is a restriction endonuclease?

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  12. Golden rice is a genetically modified crop plant where the incorporate...

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  13. An analysis of chromosomal DNA using the Southern hybridization techni...

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  14. In vitro clonal propagation in plants in characterized by:

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  15. Which vector can clone only a small fragment of DNA?

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  16. DNA fragments generated by the restriction endonucleases in a chemica...

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  17. The colonies of recombinant bacteria appear white in contrast to blue ...

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  18. For transformation, micro - particles coated with DNA to be bombarded ...

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  19. There is a restriction endonuclease called EcoR l . What does "co" par...

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  20. Agarose extracted from sea weeds finds use in:

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  21. Which one of the following is used as vector for cloning genes into hi...

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