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Given below are some step for slicing fo...

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium.
(i) Transform bacteria with recombinant
(ii) Cut the plasmid DNA using restriction enzyme .
(iii) Extract plasmid DNA to - DNA fragments DNA
(iv) Bind the plasmid DNA to non-plasmid DNA fragments .
(v) Use ligase to seal plasmid DNA to non- plasmid DNA
Arrange the above steps in the right sequence and select the CORRECT option

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I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacteria cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligate to seal plasmid DNA to non-plasmid DNA. From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacteria cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligate to seal plasmid DNA to non-plasmid DNA. From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

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