All are true about glycolysis, except

Options

A. Oxygen independent pathway
B. It produces one pyruvic acid molecule from each glucose
C. It occurs in the cytoplasm of cell
D. It can operate by using both glucose and fructose

Answer

### Step-by-Step Solution:

1. **Understanding Glycolysis**: Glycolysis is a metabolic pathway that breaks down glucose to produce energy in the form of ATP. It is a crucial process for both prokaryotic and eukaryotic organisms.

2. **Oxygen Independence**: One of the key characteristics of glycolysis is that it does not require oxygen. This means that glycolysis can occur in both aerobic (with oxygen) and anaerobic (without oxygen) conditions. Therefore, the statement that glycolysis is an oxygen-independent pathway is true.

3. **Production of Pyruvic Acid**: Glycolysis involves the breakdown of one molecule of glucose, which is a 6-carbon compound. The process results in the formation of two molecules of pyruvic acid, each of which is a 3-carbon compound. Thus, the statement that glycolysis produces one pyruvic acid molecule from each glucose is incorrect.

4. **Location of Glycolysis**: Glycolysis occurs in the cytoplasm of the cell. This is true for both prokaryotic and eukaryotic cells, as prokaryotes do not have membrane-bound organelles. Therefore, the statement regarding the location of glycolysis is correct.

5. **Substrates for Glycolysis**: Glycolysis can utilize different types of sugars, including glucose and fructose. Fructose can enter the glycolytic pathway as fructose-1,6-bisphosphate or fructose-6-phosphate. Hence, the statement that glycolysis can operate using both glucose and fructose is also true.

6. **Identifying the False Statement**: After analyzing all the statements about glycolysis, the only incorrect statement is the one that claims glycolysis produces one pyruvic acid molecule from each glucose. The correct outcome is that it produces two pyruvic acid molecules from one glucose molecule.

### Conclusion:
The answer to the question "All are true about glycolysis, except" is the statement that glycolysis produces one pyruvic acid molecule from each glucose.

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