If a probe is made of radioactively labelled dsDNA and is allowed to hybridise to its complementary DNA in a cloried cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?

Options

A. It is double stranded DNA
B. It is radioactively labelled
C. This probe can be used in autoradiography
D. It is hybridise to complementary DNA/RNA

Answer

To solve the question regarding the use of a radioactively labeled double-stranded DNA (dsDNA) probe for hybridization and autoradiography, we will analyze the statements provided and determine which one is incorrect.

### Step-by-step Solution:

1. **Understanding the Probe**: A probe is a fragment of DNA or RNA that is labeled (in this case, radioactively) and is used to detect complementary sequences in a sample. The probe hybridizes (binds) to its complementary DNA sequence.

2. **Characteristics of the Probe**:
- The probe is made of double-stranded DNA (dsDNA).
- It is labeled with a radioactive isotope, allowing for detection through autoradiography.

3. **Hybridization Process**: The dsDNA probe will hybridize to its complementary DNA sequence in the target cell. This means that one strand of the probe will bind to the complementary strand of the target DNA.

4. **Detection Method**: Autoradiography is a technique used to visualize the hybridized probe. The radioactive label on the probe emits radiation, which can be captured on photographic film or a detector, showing where the probe has bound to the DNA.

5. **Analyzing the Statements**:
- **Statement 1**: "It is a double-stranded DNA." - This statement is considered incorrect in the context of hybridization detection because while the probe is dsDNA, during the hybridization process, only one strand of the dsDNA probe binds to the complementary strand of the target DNA. The other strand does not participate in the hybridization.
- **Statement 2**: "It is radioactively labeled." - This statement is correct as the probe is indeed radioactively labeled.
- **Statement 3**: "This probe can be used in autoradiography." - This statement is also correct since autoradiography is the method used to detect the radioactively labeled probe.
- **Statement 4**: "It hybridizes to complementary DNA and RNA." - This statement is somewhat misleading; while probes can hybridize to complementary sequences, the context here is primarily about DNA. The focus should be on DNA hybridization.

6. **Conclusion**: The statement that is considered wrong is the first one, regarding the nature of the double-stranded DNA probe in the context of hybridization.

### Final Answer:
The statement that is considered wrong is: "It is a double-stranded DNA."