The main challenge for production of insulin using rDNA techniques was getting insulin into matured form using _________ bond

Options

A. Hydrogen
B. Peptide
C. Ionic
D. Disulphide

Answer

**Step-by-Step Solution:**

1. **Understanding Insulin Structure**: Insulin is a peptide hormone composed of two chains, known as chain A and chain B. Chain A consists of 21 amino acids, while chain B consists of 30 amino acids.

2. **Identifying Peptide Bonds**: The two chains of insulin are linked together by specific types of bonds. The primary challenge in producing mature insulin using recombinant DNA (rDNA) technology is ensuring that these bonds are formed correctly.

3. **Recognizing Disulfide Linkages**: The bonds that link the two chains of insulin are known as disulfide linkages. These are covalent bonds formed between the sulfur atoms of cysteine residues in the peptide chains.

4. **Challenges in rDNA Technology**: In prokaryotic systems (like bacteria), the cellular machinery does not facilitate the formation of disulfide bonds. Therefore, when insulin is produced in such systems, it does not achieve its mature, functional form.

5. **Conclusion**: The main challenge for the production of insulin using rDNA techniques was getting insulin into its matured form using disulfide bonds.

**Final Answer**: The main challenge for the production of insulin using rDNA techniques was getting insulin into matured form using **disulfide bonds**.

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