Some of the steps involved in production of humulin are given below. Choose the correct sequence
(i) Synthesis of insulin gene artificially
(ii) Culturing recombinant E. coli in bioreactors
(iii) Purification of humulin
(iv) Insertion and human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from E. coli.

Options

A. ii, i, iv, iii, v, vi
B. i, iii, v, vi, ii, iv
C. I,iv,v,ii,vi,iii
D. iii,v,ii,I,vi,iv

Answer

C