DNA Finger Printing

What is the correct sequence of DNA finger printing ? a-seperation of desired DNA by gel electrophoresis b-Digestion by restrication endonuclease c-Isolation of DNA d-Hybridisation using labelled VNTR probe e-Southern blotting

Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe. I. Hybridisation using labelled VNTR prode. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridisation DNA fragments by autoradigraphy. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

Forensic scientists have to work often with extremely small samples of DNA obtained from the hair or other sources of suspected criminals. However, it is possible to produce DNA finger print from these small samples by using :

Gel electrophoresis, the method in finger printing involves

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