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Restricito enzymes cut the strand of DNA...

Restricito enzymes cut the strand of DNA a little away from the centre of the palindromic site, but between the same two bases on the opposite single stranded strand, these overhanging stretches formed on each strand are called __________

A

Blunt ends

B

Stickly ends

C

Staggered end

D

Both (2) & (3)

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The correct Answer is:
To answer the question regarding the overhanging stretches formed by restriction enzymes, follow these steps: ### Step-by-Step Solution: 1. **Understanding Restriction Enzymes**: - Restriction enzymes are proteins that cut DNA at specific sequences known as restriction sites. These sites are often palindromic, meaning the sequence reads the same forwards and backwards on complementary strands. 2. **Identifying the Cut**: - When a restriction enzyme, such as EcoR1, recognizes a palindromic sequence, it cuts the DNA at a specific location. For example, EcoR1 cuts the sequence between the bases G and A in the sequence 5' - GaATTC - 3'. 3. **Formation of Overhangs**: - The cut made by the restriction enzyme does not occur directly in the center of the palindromic site but rather a little away from it. This results in the formation of overhanging ends on both strands of DNA. 4. **Defining the Overhanging Structures**: - The overhanging stretches that are formed on each strand after the cut are known as "sticky ends." These sticky ends are capable of base pairing with complementary sequences, making them useful in various molecular biology applications. 5. **Conclusion**: - Therefore, the answer to the question is that the overhanging stretches formed on each strand by the action of restriction enzymes are called "sticky ends." ### Final Answer: The overhanging stretches formed on each strand by restriction enzymes are called **sticky ends**.
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Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

Assertion : Restriction enzymes cut the strand of DNA a little away from the centre of the palindromic sites Reason: Restriction enzymes cut the strads of DNA between the same two bases on the opposite strand.

Restriction endonuclease ( EcoRI) enzyme cut the strand of DNA

Consider the following statements. A. Endonucleases remove nucleotides from the ends of the DNA whereas, exonucleases make cuts a specific positions within the DNA. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites. C. Asexual reproductions provides opportunities for variations and formulation of unique combinations of genetic setup. ltbrtgt D. DNA fragments can be separated by a technique known as gel electrophoresis. Of the above statements:

How are 'sticky ends' formed on a DNA strand? Why are they so called ?

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickness of the ends facilitates the action of the enzyme DNA polymerase

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA polymerase

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : In this process, there is net gain of twenty four molcules of ATP.

Number of nitrogen bases in a single strand of B-DNA with 100 turns is

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