Some of the steps involved in the production of Humulin are given below. Choose the correct sequence
I. Synthesis of gene for human insulin artificially
II. Culturing recombinant E.coli in bioreactors
III. Purification of Humulin
IV. Insertion of human insulin gene into plasmid
V. Introduction of recombinant plasmid into E.coli
VI. Extraction of recombinant gene product from E.coli.

Options

A. I, II, IV, III, V, VI
B. I,III, V, VI,II,IV
C. I, IV, V, II, VI, III
D. III, V, II, I, VI, IV

Answer

C

Explanation

I, IV, V, II, VI, III