A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem

Options

A. Red and green
B. Green and red
C. Organ and yellow
D. Purple and orange

Explanation

To determine the color of the stained xylem and phloem in a transverse section of a stem that has been stained first with safranin and then with fast green, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Staining Process**: The process involves two different stains: safranin and fast green. Each stain has a specific color and targets different components of the plant tissue.

2. **Identify the Colors of the Stains**:
- **Safranin**: This stain is red in color.
- **Fast Green**: This stain is green in color.

3. **Know the Target Components**:
- **Safranin**: It primarily stains lignified cell walls and chromatin (nuclei) red. Lignified cells are found in xylem.
- **Fast Green**: This stain targets cellulose cell walls and cytoplasm, staining them green. Phloem contains these types of cells.

4. **Apply the Stains to Xylem and Phloem**:
- **Xylem**: Since xylem has lignified walls, it will be stained red by safranin.
- **Phloem**: Phloem contains cells with cellulose walls and cytoplasm, which will be stained green by fast green.

5. **Conclude the Colors**:
- The xylem will appear red due to the safranin stain.
- The phloem will appear green due to the fast green stain.

### Final Answer:
- The color of the stained xylem will be **red** and the color of the stained phloem will be **green**.

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