To isolate protoplast, one needs

Options

A. Pectinase
B. Cellulase
C. both pecthase cellulase
D. chitnase

Explanation

To isolate protoplast, one needs the following steps:

1. **Understanding Protoplast**: Protoplasts are the living part of a plant or bacterial cell, which includes the cytoplasm and organelles, but without the cell wall.

2. **Components of the Cell Wall**: The plant cell wall is primarily composed of pectin and cellulose. To isolate the protoplast, these components need to be removed.

3. **Enzymatic Digestion**: To break down the cell wall, specific enzymes are used. The two key enzymes required for this process are:
- **Pectinase**: This enzyme breaks down pectin, a polysaccharide found in the plant cell wall.
- **Cellulase**: This enzyme breaks down cellulose, another major component of the cell wall.

4. **Combination of Enzymes**: The isolation of protoplasts requires a combination of both pectinase and cellulase. This combination effectively digests the cell wall, allowing the protoplast to be isolated.

5. **Conclusion**: Therefore, to isolate protoplasts, one needs both pectinase and cellulase.

**Final Answer**: The correct answer is option 3: both pectinase and cellulase.

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