what are the physical barriers of a cell in the protoplast fusion experiment?How are these barriers overcome ?

Explanation

### Step-by-Step Solution:

1. **Identify the Physical Barrier**:
- The primary physical barrier of a cell in the protoplast fusion experiment is the **cell wall**. The cell wall provides structural support and protection to the cell.

2. **Understanding Protoplast Fusion**:
- Protoplast fusion involves the merging of two plant cells by removing their cell walls, allowing their protoplasts (the living part of the cell, including the cell membrane and cytoplasm) to fuse together.

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