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The most important feature in a plasmid ...

The most important feature in a plasmid to be used as a vector is

A

Origin of replication (Ori)

B

presence of a selectable marker

C

presence of sites for restriction endonuclease

D

its size

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### Step-by-Step Solution: 1. **Understanding the Role of Plasmids**: Plasmids are small, circular DNA molecules found in bacteria that can replicate independently of chromosomal DNA. They are commonly used as vectors in genetic engineering to transfer genetic material into host cells. 2. **Identifying Key Features of Plasmids**: Several features are essential for a plasmid to function effectively as a vector: - **Origin of Replication (ORI)**: This is a specific DNA sequence that allows the plasmid to replicate within the host cell. - **Selectable Marker**: This feature enables the identification of cells that have successfully taken up the plasmid. - **Restriction Endonuclease Sites**: These are specific sequences where enzymes can cut the DNA, allowing for the insertion of foreign DNA. ...
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NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
  1. Which of the following is not required in the preparation of a recombi...

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  2. In agarose gel electrophoresis, DNA molecules are separated on the bas...

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  3. The most important feature in a plasmid to be used as a vector is

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  4. While isolating DNA from bacteria, which of the following enzymes is n...

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  5. Which of the following has popularised the PCR (polymerase chain react...

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  6. An antibiotic resistance gene in a vector usually helps in the selecti...

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  7. Significance of 'heat shock' method in bacterial transformation is to ...

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  8. The role of DNA ligase in the construction of a recombinant DNA molec...

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  9. Which of the following is not a source of restriction endonuclease ?

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  10. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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  11. A bacterial cell was transformed with a recombinant DNA that was gener...

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  12. Which of the following should be chosen for best yield if one were to ...

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  13. Who among the following was awarded the Nobal Prize for the developmen...

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  14. Which of the following statements does not hold true for restriction e...

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  15. How is copy number of the plasmid vector related to yield of recombina...

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  16. Would you choose an exonuclease, while producing a recombinant DNA mol...

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  17. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  18. Restriction enzymes should not have more than one site of action in th...

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  19. What does competent refer to in competent cells used in transformation...

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  20. What is the significance of adding proteases at the time of isolation ...

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