While isolating DNA from bacteria, which of the following enzymes is not used ?

Options

A. Lysozyme
B. Ribonuclease
C. Deoxyribonuclease
D. Protease.

Explanation

To determine which enzyme is not used while isolating DNA from bacteria, we can analyze the functions of the enzymes listed in the options. Here's a step-by-step breakdown of the reasoning:

### Step 1: Understand the Purpose of DNA Isolation
When isolating DNA from bacteria, the goal is to extract pure DNA without contamination from other cellular components such as proteins and RNA.

### Step 2: Evaluate Each Enzyme
1. **Lysozyme**: This enzyme is used to break down the bacterial cell wall, facilitating the release of DNA. Therefore, it is used in the isolation process.

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