Which of the following has popularised the PCR (polymerase chain reaction)? (a) Easy availability of DNA template (b) Availability of synthetic primers (c) Availability of cheap deoxyribonucleotides (d) Availability of ‘Thermostable’ DNA polymerase

Options

A. Easy availability of DNA template
B. Availability of synthetic primeres
C. Availability of cheap deoxyribonucleotides.
D. Availability of Thermostable DNA polymerase

Explanation

To determine which factor has popularized the Polymerase Chain Reaction (PCR), we need to analyze each of the provided options:

1. **Understanding PCR**: PCR is a technique used to amplify DNA, making millions of copies of a specific DNA segment. The process involves three main steps: denaturation, annealing, and extension.

2. **Denaturation**: The first step involves heating the DNA to about 96 degrees Celsius, which separates the two strands of the DNA helix.

3. **Annealing**: The temperature is then lowered to around 55 degrees Celsius to allow synthetic primers to bind to the target DNA sequence.

...