Home
Class 12
BIOLOGY
Which of the following steps are catalys...

Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?

A

Denaturation of template DNA.

B

Annealing of primeres to template DNA

C

Extension of primer end on the template DNA

D

All of the above.

Text Solution

AI Generated Solution

### Step-by-Step Solution: 1. **Understanding PCR**: - PCR stands for Polymerase Chain Reaction, which is a technique used to amplify a specific segment of DNA. The process involves repeated cycles of heating and cooling to facilitate the replication of DNA. 2. **Role of Taq Polymerase**: - Taq polymerase is a thermostable enzyme derived from the bacterium Thermus aquaticus. It is crucial for the PCR process because it can withstand the high temperatures used during the reaction without denaturing. ...
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY AND ITS APPLICATIONS

    NCERT EXEMPLAR ENGLISH|Exercise All Questions|57 Videos
  • ECOSYSTEM

    NCERT EXEMPLAR ENGLISH|Exercise Long Answer Type Question|12 Videos

Similar Questions

Explore conceptually related problems

Which of the following steps is/are catalysed by Taq polymerase in a PCR ?

Which of the following steps in transcription is catalysed by RNA polymerase?

Which of the following steps in transcription is catalysed by RNA polymerase?

Which of the following is the correct sequence of PCR (polymerase chain reaction)?

Which of he following is'the correct sequence of PCR or polymerase chain reaction

Which of the following has popularised the PCR (polymerase chain reactions)?

Which of the following has popularised the PCR (polymerase chain reactions)?

Which of the following has popularised the PCR (polymerase chain reaction)? (a) Easy availability of DNA template (b) Availability of synthetic primers (c) Availability of cheap deoxyribonucleotides (d) Availability of ‘Thermostable’ DNA polymerase

NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
  1. The role of DNA ligase in the construction of a recombinant DNA molec...

    Text Solution

    |

  2. Which of the following is not a source of restriction endonuclease ?

    Text Solution

    |

  3. Which of the following steps are catalysed by Taq polymerase in a PCR ...

    Text Solution

    |

  4. A bacterial cell was transformed with a recombinant DNA that was gener...

    Text Solution

    |

  5. Which of the following should be chosen for best yield if one were to ...

    Text Solution

    |

  6. Who among the following was awarded the Nobal Prize for the developmen...

    Text Solution

    |

  7. Which of the following statements does not hold true for restriction e...

    Text Solution

    |

  8. How is copy number of the plasmid vector related to yield of recombina...

    Text Solution

    |

  9. Would you choose an exonuclease, while producing a recombinant DNA mol...

    Text Solution

    |

  10. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

    Text Solution

    |

  11. Restriction enzymes should not have more than one site of action in th...

    Text Solution

    |

  12. What does competent refer to in competent cells used in transformation...

    Text Solution

    |

  13. What is the significance of adding proteases at the time of isolation ...

    Text Solution

    |

  14. While doing a PCR, denaturation step is missed. What will be its effec...

    Text Solution

    |

  15. Name a recomblnant vaccine that is currently being used in vaccination...

    Text Solution

    |

  16. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

    Text Solution

    |

  17. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

    Text Solution

    |

  18. What is meant by gene cloning?

    Text Solution

    |

  19. Both a wine maker and a molecular biologist who had developed a recomb...

    Text Solution

    |

  20. A recombinant DNA molecule was created by ligating a gene to a plasmid...

    Text Solution

    |