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Would you choose an exonuclease, while p...

Would you choose an exonuclease, while producing a recombinant DNA molecule?

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**Step-by-Step Solution:** 1. **Understanding Exonucleases**: Exonucleases are enzymes that remove nucleotide units from the ends of a DNA molecule. They work by cleaving the phosphodiester bonds at the free ends of the DNA. 2. **Impact on DNA Fragments**: If exonucleases are used during the production of recombinant DNA, they would shorten the DNA fragments by removing nucleotides from the ends. This can lead to the complete degradation of the DNA fragment that contains the gene of interest. 3. **Sticky Ends vs. Blunt Ends**: The goal in producing recombinant DNA is often to create sticky ends, which are overhanging sequences that can easily anneal with complementary sequences. Exonucleases do not create sticky ends; instead, they create blunt ends or degrade the DNA entirely. ...
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Read the following statements and select the correct ones. (i) Electrophoresis is a technique used for the separation of molecules based on their size and charge. (ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast,. (iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule. (iv) In EcoRl, the roman numberal I indicates that it was the first enzyme isolated from E.coli A) (i) and (ii) B) (iii) and (iv) C) (i),(ii) and (iv) D) (i),(ii),(iii) and (iv)

Who produced first recombinant DNA molecule?

In the DNA molecule

In the DNA molecule

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What is recombinant DNA?

The cell which contain recombinant DNA

First artificial recombinant DNA molecule was created by:

The role of DNA ligase in the construction of a recombinant DNA molecule is :

NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
  1. Which of the following statements does not hold true for restriction e...

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  2. How is copy number of the plasmid vector related to yield of recombina...

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  3. Would you choose an exonuclease, while producing a recombinant DNA mol...

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  4. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  5. Restriction enzymes should not have more than one site of action in th...

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  6. What does competent refer to in competent cells used in transformation...

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  7. What is the significance of adding proteases at the time of isolation ...

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  8. While doing a PCR, denaturation step is missed. What will be its effec...

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  9. Name a recomblnant vaccine that is currently being used in vaccination...

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  10. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  11. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  12. What is meant by gene cloning?

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  13. Both a wine maker and a molecular biologist who had developed a recomb...

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  14. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  15. Restriction enzymes that are used in the construction of recombinant D...

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  16. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  17. How does one visualize DNA on an agarose gel?

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  18. A plasmid without a selectable marker was chosen as vector for cloning...

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  19. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  20. Describe the role of CaCl(2) in the preparation of competent cells?

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