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Restriction enzymes should not have more...

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

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### Step-by-Step Solution: 1. **Understanding Restriction Enzymes**: - Restriction enzymes, also known as molecular scissors, are proteins that cut DNA at specific sequences. They are classified as nucleases, which can be further divided into exonucleases and endonucleases. Exonucleases cut DNA at the ends, while endonucleases cut at specific internal sites. 2. **Cloning Vectors**: - Cloning vectors are DNA molecules used to transport foreign genetic material into a host cell. They typically contain a cloning site where foreign DNA can be inserted. ...
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NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
  1. Would you choose an exonuclease, while producing a recombinant DNA mol...

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  2. What does H in 'Hind' and III refer to the enzyme 'Hind III'?

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  3. Restriction enzymes should not have more than one site of action in th...

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  4. What does competent refer to in competent cells used in transformation...

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  5. What is the significance of adding proteases at the time of isolation ...

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  6. While doing a PCR, denaturation step is missed. What will be its effec...

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  7. Name a recomblnant vaccine that is currently being used in vaccination...

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  8. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  9. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  10. What is meant by gene cloning?

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  11. Both a wine maker and a molecular biologist who had developed a recomb...

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  12. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  13. Restriction enzymes that are used in the construction of recombinant D...

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  14. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  15. How does one visualize DNA on an agarose gel?

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  16. A plasmid without a selectable marker was chosen as vector for cloning...

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  17. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  18. Describe the role of CaCl(2) in the preparation of competent cells?

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  19. What would happen when one grows a recombinant bacterium in the biorea...

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  20. Identify and explain A,B and C in the PCR diagram given below.

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