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What is the significance of adding prote...

What is the significance of adding proteases at the time of isolation of genetic material (DNA)?

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**Step-by-Step Solution:** 1. **Understanding the Purpose of DNA Isolation**: The primary goal of isolating DNA is to obtain a purified form of genetic material. This purified DNA is essential for various applications in biotechnology and genetic engineering. 2. **Importance of Purity**: For DNA to be useful in experiments and applications, it must be free from contaminants, particularly macromolecules like proteins, lipids, and carbohydrates. The presence of these contaminants can interfere with subsequent processes such as cloning, sequencing, or analysis. 3. **Role of Proteases**: Proteases are enzymes that specifically break down proteins into smaller peptides or amino acids. By adding proteases during the DNA isolation process, we can effectively degrade any proteins that may be associated with the DNA. ...
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NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
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  2. What does competent refer to in competent cells used in transformation...

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  4. While doing a PCR, denaturation step is missed. What will be its effec...

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  5. Name a recomblnant vaccine that is currently being used in vaccination...

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  6. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  7. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  8. What is meant by gene cloning?

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  9. Both a wine maker and a molecular biologist who had developed a recomb...

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  10. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  11. Restriction enzymes that are used in the construction of recombinant D...

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  12. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  13. How does one visualize DNA on an agarose gel?

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  14. A plasmid without a selectable marker was chosen as vector for cloning...

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  15. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  16. Describe the role of CaCl(2) in the preparation of competent cells?

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  17. What would happen when one grows a recombinant bacterium in the biorea...

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  18. Identify and explain A,B and C in the PCR diagram given below.

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  19. Name the regions marked A,B and C

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  20. For selection of recombinants, insertional inactivation of antibiotic ...

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