While doing a PCR, denaturation step is missed. What will be its effect on the process?

Explanation

### Step-by-Step Solution:

1. **Understanding PCR Process**: PCR, or Polymerase Chain Reaction, is a technique used to amplify a specific segment of DNA. It involves three main steps: denaturation, annealing, and elongation.

2. **Denaturation Step**: The first step, denaturation, involves heating the double-stranded DNA to about 94-95°C. This heat causes the hydrogen bonds between the base pairs to break, resulting in the separation of the DNA into two single strands.

3. **Consequences of Missing Denaturation**: If the denaturation step is missed, the double-stranded DNA will remain intact. This means that the DNA will not be separated into single strands.

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