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What is meant by gene cloning?...

What is meant by gene cloning?

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### Step-by-Step Solution to the Question: What is meant by gene cloning? 1. **Definition of Gene Cloning**: Gene cloning is a biotechnological process that involves creating multiple copies of a specific gene. This is done to produce a large number of identical copies of the gene of interest. 2. **Isolation of the Gene**: The first step in gene cloning is to isolate the gene of interest from a host organism. This involves extracting the DNA that contains the desired gene. 3. **Insertion into a Vector**: Once the gene of interest is isolated, it is then ligated (joined) into a vector. A vector is a DNA molecule that can carry foreign DNA into a host cell. Common vectors include plasmids. ...
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NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
  1. What is the significance of adding proteases at the time of isolation ...

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  2. While doing a PCR, denaturation step is missed. What will be its effec...

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  3. Name a recomblnant vaccine that is currently being used in vaccination...

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  4. Do biomolecules (DNA and protein) exhibit biological activity in anhyd...

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  5. What modification is done on the Ti-plasmid of Agrobacterium tumefacie...

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  6. What is meant by gene cloning?

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  7. Both a wine maker and a molecular biologist who had developed a recomb...

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  8. A recombinant DNA molecule was created by ligating a gene to a plasmid...

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  9. Restriction enzymes that are used in the construction of recombinant D...

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  10. A plasmid DNA and linear DNA (both are of the same size) have one site...

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  11. How does one visualize DNA on an agarose gel?

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  12. A plasmid without a selectable marker was chosen as vector for cloning...

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  13. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  14. Describe the role of CaCl(2) in the preparation of competent cells?

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  15. What would happen when one grows a recombinant bacterium in the biorea...

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  16. Identify and explain A,B and C in the PCR diagram given below.

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  17. Name the regions marked A,B and C

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  18. For selection of recombinants, insertional inactivation of antibiotic ...

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  19. Describe the role of agrobacterium in transforming a plant cell.

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  20. Illustrate the design of a bioreactor. Highlight the difference betwee...

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