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A recombinant DNA molecule was created b...

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake. An exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial trasformation?

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**Step-by-Step Solution:** 1. **Understanding the Creation of Recombinant DNA**: A recombinant DNA molecule is formed by ligating a gene of interest to a plasmid vector. This process results in the formation of a circular and closed DNA structure. 2. **Role of Exonuclease**: Exonucleases are enzymes that degrade nucleic acids by removing nucleotides from the ends of DNA. They require an open end to attach and begin their action. 3. **Impact of Exonuclease on Circular DNA**: Since the recombinant DNA is circular and closed, it does not have any open ends. Therefore, the exonuclease cannot bind to the DNA to degrade it. ...
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NCERT EXEMPLAR ENGLISH-BIOTECHNOLOGY PRINCIPLES AND PROCESS-All Questions
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  11. How does one visualize DNA on an agarose gel?

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  13. A mixture of fragmented DNA was electrophoresed in an agarose gel. Af...

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  14. Describe the role of CaCl(2) in the preparation of competent cells?

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  16. Identify and explain A,B and C in the PCR diagram given below.

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  17. Name the regions marked A,B and C

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