Mark the incorrect statement in context to `O_(2)` binding to Hb 

Options

A. lower pH
B. lower temperature
C. lower `pCO_(2)`
D. higher `pO_(2)`

Explanation

To determine the incorrect statement regarding the binding of oxygen (O₂) to hemoglobin (Hb), we need to analyze the options provided:

1. **Lower pH**: When the pH decreases (becomes more acidic), hemoglobin's affinity for oxygen decreases. This is known as the Bohr effect, where increased hydrogen ion concentration (lower pH) promotes the release of oxygen from hemoglobin. This statement is correct.

2. **Lower temperature**: A decrease in temperature increases hemoglobin's affinity for oxygen, allowing it to hold onto oxygen more tightly. This statement is also correct.

3. **Lower partial pressure of CO₂**: A lower partial pressure of carbon dioxide (CO₂) leads to an increase in hemoglobin's affinity for oxygen. This is because lower CO₂ levels result in higher pH (less acidity), which promotes oxygen binding. This statement is correct.

4. **Higher partial pressure of O₂**: An increase in the partial pressure of oxygen enhances the binding of oxygen to hemoglobin. This is a fundamental principle of gas exchange, where higher concentrations of oxygen drive the binding process. However, this statement is misleading in the context of the question as it is not incorrect in a general sense, but rather it is less relevant compared to the other factors mentioned.

Based on the analysis, the incorrect statement in the context of O₂ binding to hemoglobin is:

**Correct Answer: Higher partial pressure of O₂ (Option 4)**