While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine=29 %, Guanine= 17%, Cytosine=32%, Thymine=17 %, Considering the Chargaff's rule it can be concluded that

Options

A. it is a double-stranded circular DNA
B. it is single-stranded DNA
C. it is a double-stranded linear DNA
D. NO conclusion can be drawn

Explanation

To solve the question regarding the analysis of DNA and its adherence to Chargaff's rule, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Chargaff's Rule**: Chargaff's rule states that in double-stranded DNA, the amount of adenine (A) is equal to thymine (T) and the amount of guanine (G) is equal to cytosine (C). This means:
- A = T
- G = C

2. **Analyzing the Given Data**: We have the following proportions of nucleotides:
- Adenine (A) = 29%
- Thymine (T) = 17%
- Guanine (G) = 17%
- Cytosine (C) = 32%

3. **Checking the A = T Condition**: According to Chargaff's rule, the percentage of adenine should equal the percentage of thymine. Here:
- A = 29%
- T = 17%
- Since 29% ≠ 17%, this condition is not met.

4. **Checking the G = C Condition**: Now, we check if the percentage of guanine equals the percentage of cytosine:
- G = 17%
- C = 32%
- Since 17% ≠ 32%, this condition is also not met.

5. **Conclusion**: Since neither of the conditions of Chargaff's rule is satisfied, we can conclude that the DNA analyzed does not follow the rules applicable to double-stranded DNA. Therefore, it indicates that the DNA is likely single-stranded.

### Final Conclusion:
The DNA analyzed is single-stranded DNA, as it does not adhere to Chargaff's rule.

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