Discuss the singnificance of heavy isotope of nitrogen in the Meselson and Stahl's experiment .

Explanation

### Step-by-Step Text Solution

1. **Introduction to Meselson and Stahl's Experiment**:
- The Meselson and Stahl experiment aimed to determine the mechanism of DNA replication. They specifically wanted to prove that DNA replication is semi-conservative.

2. **Use of Heavy Isotope of Nitrogen**:
- The researchers used a heavy isotope of nitrogen, Nitrogen-15 (15N), to label the DNA. They grew E. coli bacteria in a medium containing ammonium chloride (NH4Cl) enriched with 15N for several generations. This allowed the bacteria to incorporate the heavy nitrogen into their DNA.

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