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Discuss the singnificance of heavy isoto...

Discuss the singnificance of heavy isotope of nitrogen in the Meselson and Stahl's experiment .

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### Step-by-Step Text Solution 1. **Introduction to Meselson and Stahl's Experiment**: - The Meselson and Stahl experiment aimed to determine the mechanism of DNA replication. They specifically wanted to prove that DNA replication is semi-conservative. 2. **Use of Heavy Isotope of Nitrogen**: - The researchers used a heavy isotope of nitrogen, Nitrogen-15 (15N), to label the DNA. They grew E. coli bacteria in a medium containing ammonium chloride (NH4Cl) enriched with 15N for several generations. This allowed the bacteria to incorporate the heavy nitrogen into their DNA. ...
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Knowledge Check

  • Watson and Crick (1953) proposed DNA was based on (i) X-ray diffraction studies studies of DNA dne by Wilkins and Franklin (ii) Chargaff's base equivalence rule (iii)Griffith's transformation experiment (iv) Meselson and Stahl's experiment.

    A
    (i),(ii) and (iv)
    B
    (i) and (ii)
    C
    (iii) and (iv)
    D
    (i), (ii),(iii) and (iv)

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    Answer the following questions based on Meselson and Stahl s experiment: (a) Write the name of the chemical substance used as a source of nitrogen in the experiment by them. (b) Why did the scientists synthesise the light and the heavy DNA molecules In the organism used in the experiment ? (c) How did the scientists make it possible to distinguish the heavy DNA molecule from the light DNA molecule ? Explain. ((1) Write the conclusion the scientists arrived at after completing the experiment.

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