Describe the roles of (a) high temperature, (b) primers, and (c ) bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.

Describe the roles of heat , primers and the bacterium Thermus aquaticus in the process of PCR.

Which type of primer is used the genetic engineering tool -polymerase chain Reaction?

Which of the following statement are correct for the enzyme Taq polymerase? (i) It remains active during the high temperature . (ii) It requies primers for carrying out the process of polymerization. (iii) It induces denaturation of double-stranded DNA.

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the high temperature induced denaturation of dsDNA. (ii) It requires primers for carrying out the process of polymerisation. (iii) It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

Which of these statements about Taq polymerase used during PCR is not correct ? (a) It is used for primer extension to synthesise a new DNA strand (b) It is a thermostable enzyme and can survive temperatures up to 125 ∘ C (c) It is obtained from Thermus aquaticus (d) Its addition eliminated the need to add fresh polymerase in every PCR cycle

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Food material can be preserved at (a) High temperature (b) Low temperature (c) Osmotic temperature (d) All the above.

In the three steps (a,b,c) of polymerase chain reaction, select the correct step

For the reaction I_(2(g)) hArr I_(2(s)), Delta H= -ve . Then choose the correct statement from the following (A) The process is spontaneous at all temperature (B) The process is accompained by an increase in entropy (C ) The process is accompained by a decrease in entropy (D) The process is accompained by a decrease in enthalpy

Taq polymerase used in polymerase chain reaction is active at temperature of