How does `beta`-galactosidase coding sequence act as a selectable marker ? Why is it a preferred selectable marker to antibiotic resistance genes ? Explain.

Why is it essential to have a ' selectable marker' in a cloning vector

(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play. (b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

Genes for which of the following can serve as selectable marker ?

Which of the following is the first artificial cloning vector that has two selectable markers—tetracycline (tetR) and antibiotic restriction enzymes (ampR)?

How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

(a) Identify the selectable markers in the diagram of E. Coli vectior shown above . ( b) How is the coding squence of alpha - galactosidase consider a better markers than the ones identified by you in the diagram ? Explain.

A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?

A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?