In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be :

If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid u ill:

If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid will:

Resistance genes present in pBR322 are

The usual source of restriction endonucleases used in gene clonning is

If the gene of interest is introduced in a tetracycline - resistant gene region of pBR322, then: (a) The host will become resistant for tetracycline (b) The host will start producing tetracycline (c) The host will start producing ampicillin. (d) The host will loose resistance for antibiotic tetracycline.

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain

A plasmid DNA and linear DNA (both are of the same size) have one site for restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band, while linear DNA shows two fragments Explain.

Why are only type II restriction endonucleases used in recombinant DNA technology?

Assertion (A) : Restriction endonucleases recognise short pallindromic sequence and cut at specific sites Reason (R) : When a restriction endonuclease acts on a pallindrome, it cleaves both the strands of DNA molecule.

In pBR322, tetracycline resistance gene (tet^(R)) has recognition site for which of the following restriction endonuclease ? 1) Hindlll 2) BamHl 3) EcoRl 4) Pstl