(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play.
(b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

(a) Identify the selectable markers in the diagram of E. Coli vectior shown above . ( b) How is the coding squence of alpha - galactosidase consider a better markers than the ones identified by you in the diagram ? Explain.

Why is it essential to have a ' selectable marker' in a cloning vector

How does beta -galactosidase coding sequence act as a selectable marker ? Why is it a preferred selectable marker to antibiotic resistance genes ? Explain.

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

Mention the rule of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.

Explain the role(s) of the following Biotechnology: a) Restriction endonuclease Gel-electrophoresis c) Selectable markers in pBR322.

(i) Name the organism in which the vector shown in inserted to get the copies of the desired gene. (ii) Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene. (iii) Name and explain the role of a selectable marker in the vector shown.

Identify A,B,C, and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining. 1) Both statements 1 and 2 are correct 2) statement 1 is correct but statement 2 is incorrect 3) statement 1 is incorrect but statement 2 is correct 4) None of the above