Explain the various steps involved in the production of artificial insulin

Explanation

Insulin used for diabetes was earlier exlier extracted from pancreas of slaughtered cattle and pigs. Insulin consists of two short polypeptide chains A and chain B that are linked together by disulphide bridges . In mammals including humans , insulin is synthesised as aprohormone (like a pro - enzyme the pro - hormone ) also need to be processed before it becomes a fully mature and functional hormone.
In America , a company prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains . Chains A and B were produced separately , extracted and combined by creating disulfide bonds to form human insulin.