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(a) State the role of DNA ligase in biot...

(a) State the role of DNA ligase in biotechnology.
(b) What happens when Meloidegyne incognitia consumes cells with RNAi gene?

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Role of DNA ligase in biotechnology: DNA ligase helps in joining of DNA fragments end to end, having same kind of sticky ends.
(b) If Meloidegyne incognitia consume cells with RNA igene then silencing of specific mRNA occurs, due to a complementary dsRNA molecule formation. This ds RNA binds to and prevents translation of mRNA (silencing) and thus, causing death of the nematode.
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Assertion: DNA ligase plays an important role in recombinant DNA technology. Reason: The linking of antibiotic resistance gene with plasmid vector became possible by enzyme DNA ligase