When we homogenise any tissue in an acid the acid soluble pool represents

Options

A. cytoplasm
B. cell membrane
C. nucleus
D. mitochondria.

Explanation

To answer the question "When we homogenise any tissue in an acid, the acid soluble pool represents," we can break down the process and the concepts involved.

### Step-by-Step Solution:

1. **Understanding Homogenization**:
- When we homogenize a tissue, we are breaking it down into smaller components. This process involves using a homogenizer to physically disrupt the cells, allowing the contents to be released into a solution.

2. **Separation of Fractions**:
- After homogenization, the mixture can be separated into two main fractions based on solubility in acid:
- **Acid-Soluble Pool**: This fraction contains smaller molecules that dissolve in the acid.
- **Acid-Insoluble Pool**: This fraction contains larger macromolecules that do not dissolve in the acid.

3. **Components of the Acid-Soluble Pool**:
- The acid-soluble pool primarily consists of small molecules and metabolites. These include:
- Amino acids
- Sugars
- Nucleotides
- These molecules typically have a molecular weight less than 800 Daltons.

4. **Components of the Acid-Insoluble Pool**:
- The acid-insoluble pool contains larger macromolecules, such as:
- Proteins
- Nucleic acids
- Cell organelles
- These macromolecules generally have a molecular weight greater than 800 Daltons.

5. **Conclusion**:
- Therefore, when we homogenize tissue in an acid, the acid-soluble pool represents the cytoplasm and smaller molecules that are soluble in the acid.

### Final Answer:
The acid soluble pool represents the cytoplasm and smaller molecules that are soluble in the acid.

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