The most likely method, used to determine the structural details of a cell organelle is

Options

A. autoradiography
B. microdissection
C. electron microscopy
D. phase contrast microscopy.

Explanation

**Step-by-Step Text Solution:**

1. **Understanding the Question:**
The question asks for the most likely method used to determine the structural details of a cell organelle.

2. **Identifying the Options:**
The options provided are:
- Autoradiography
- Microdissection
- Electron Microscopy
- Phase Contrast Microscopy

3. **Analyzing Each Method:**
- **Autoradiography:** This technique is primarily used to visualize the distribution of radioactive materials in cells and tissues, not specifically for structural details.
- **Microdissection:** This method involves the dissection of cells or tissues under a microscope. While it can be useful for isolating specific parts, it does not provide detailed structural images.
- **Electron Microscopy:** This is a powerful imaging technique that uses electrons to create an image of the specimen. It has a very high magnification (up to 250,000 to 400,000 times) and a resolving power that is about 1000 times greater than that of a light microscope, allowing for detailed visualization of cell organelles.
- **Phase Contrast Microscopy:** This method enhances contrast in transparent specimens but is less effective for resolving fine structural details compared to electron microscopy.

4. **Conclusion:**
Based on the analysis, the most suitable method for determining the structural details of a cell organelle is **Electron Microscopy**. This technique provides high magnification and excellent resolving power, making it ideal for studying the intricate structures of cell organelles.

**Final Answer:**
The most likely method used to determine the structural details of a cell organelle is **Electron Microscopy**.

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