Select the incorrect statement.

Options

A. Amoeba and Paramecium reproduce by binary fission.
B. Buds are produced due to unequal divison in parent body
C. Encystation refers to the formation of two layered hard covering around Amoeba during unfavourable condition.
D. Spores are formed due to multiple fission in sporulation.

Explanation

To determine the incorrect statement from the given options, we will analyze each statement one by one:

1. **Statement 1**: "Amoeba and paramecium reproduce by binary fission."
- **Analysis**: This statement is true. Both amoeba and paramecium primarily reproduce through binary fission. Amoeba can also undergo multiple fission under unfavorable conditions, but the statement is still correct as it mentions binary fission.

2. **Statement 2**: "Buds are produced due to unequal division in parent body."
- **Analysis**: This statement is incorrect. Budding is a form of asexual reproduction where a new organism develops from an outgrowth or bud on the parent organism. This process does not involve the unequal division of the parent body; rather, the bud grows out from the parent and eventually detaches.

3. **Statement 3**: "Incestation refers to the formation of a two-layered hard covering around amoeba during unfavorable conditions."
- **Analysis**: This statement is also incorrect. Incestation is the process where amoeba forms a protective covering during unfavorable conditions, but it actually forms a three-layered covering, not a two-layered one. The pseudopodia degenerate to form this protective covering.

4. **Statement 4**: "Spores are formed due to multiple fission in sporulation."
- **Analysis**: This statement is misleading. Spores are typically formed through sporulation, which is a process associated with multicellular organisms like fungi. Multiple fission in unicellular organisms like amoeba leads to the formation of daughter cells, not spores.

**Conclusion**: The incorrect statements are Statement 2 and Statement 3. However, if we are to select only one incorrect statement, Statement 2 is the clearest incorrect statement regarding the process of budding.

### Step-by-Step Solution:
1. Analyze each statement regarding reproduction in organisms.
2. Confirm the accuracy of the first statement about amoeba and paramecium.
3. Identify the incorrect nature of the second statement regarding budding.
4. Clarify the misunderstanding in the third statement about incestation.
5. Discuss the confusion in the fourth statement regarding spores and multiple fission.