The anther wall consists of four wall layers where

Options

A. tapetum lies just inner to endothecium
B. middle layers lie between endothecium and tapetum
C. endothecium lies inner to middle layers
D. tapetum lies next to epidermis

Answer

### Step-by-Step Solution:

1. **Identify the Layers of the Anther Wall**:
The anther wall consists of four distinct layers. These layers are:
- Epidermis (outermost layer)
- Endothecium (second layer)
- Middle layer (between endothecium and tapetum)
- Tapetum (innermost layer)

2. **Understanding the Position of Each Layer**:
- The **epidermis** is the outermost layer that provides protection.
- The **endothecium** lies just beneath the epidermis and also serves a protective function.
- The **middle layer** is located between the endothecium and the tapetum.
- The **tapetum** is the innermost layer that provides nourishment to the developing pollen grains.

3. **Analyzing the Options**:
- **Option 1**: "Tapetum lies just inner to endothecium" - This is incorrect because the tapetum is the innermost layer, not just inner to the endothecium.
- **Option 2**: "Middle layers lie between endothecium and tapetum" - This is correct as the middle layer is indeed situated between these two layers.
- **Option 3**: "Endothecium lies inner to middle layers" - This is incorrect because the endothecium is outer to the middle layer.
- **Option 4**: "Tapetum lies next to epidermis" - This is incorrect as the tapetum is the innermost layer, while the epidermis is the outermost layer.

4. **Conclusion**:
Based on the analysis of the layers and their positions, the correct answer is:
- **Middle layers lie between endothecium and tapetum**.