To isolate protoplast, one needs

Options

A. pectinase
B. cellulase
C. both pectinase and cellulase
D. chitinase

Answer

To isolate protoplasts from plant cells, follow these steps:

### Step-by-Step Solution:

1. **Understand Protoplasts**: Protoplasts are plant cells that have had their cell walls removed. This is the first step in isolating them, as the cell wall is a barrier that must be eliminated.

2. **Identify the Composition of Cell Walls**: The cell wall is primarily made up of polysaccharides, including cellulose and pectin. Understanding this composition is crucial for knowing which enzymes to use for digestion.

3. **Select the Appropriate Enzymes**: To remove the cell wall, specific enzymes are required. The two key enzymes are:
- **Cellulase**: This enzyme breaks down cellulose, which is a major component of the cell wall.
- **Pectinase**: This enzyme breaks down pectin, another important component of the cell wall.

4. **Combine the Enzymes**: Since both cellulose and pectin are present in the cell wall, both cellulase and pectinase need to be used together to effectively digest the cell wall and isolate the protoplasts.

5. **Conclusion**: Therefore, to isolate protoplasts, one needs both pectinase and cellulase. The answer to the question is that both enzymes are required.

### Final Answer:
To isolate protoplasts, one needs **both pectinase and cellulase**.

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