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Which of the following correctly depicts...

Which of the following correctly depicts the recognition site for EcoRI ?
(a) `{:(G-A-Aoverset(darr)-T-T-C),(C-T-Tunderset(uarr)-A-A-G):}`
(b) `{:(G-T-Coverset(darr)-G-A-C),(C-A-Gunderset(uarr)-C-T-G):}`
(c) `{:(Goverset(darr)-T-C-G-A-C),(C-A-G-C-Tunderset(uarr)-G):}`
(d) `{:(Goverset(darr)-A-A-T-T-C),(C-T-T-A-Aunderset(uarr)-G):}`

A

`{:(G-A-Aoverset(darr)-T-T-C),(C-T-Tunderset(uarr)-A-A-G):}`

B

`{:(G-T-Coverset(darr)-G-A-C),(C-A-Gunderset(uarr)-C-T-G):}`

C

`{:(Goverset(darr)-T-C-G-A-C),(C-A-G-C-Tunderset(uarr)-G):}`

D

`{:(Goverset(darr)-A-A-T-T-C),(C-T-T-A-Aunderset(uarr)-G):}`

Text Solution

AI Generated Solution

The correct Answer is:
To determine which option correctly depicts the recognition site for EcoRI, we need to analyze the characteristics of EcoRI and its recognition site. ### Step-by-Step Solution: 1. **Understanding EcoRI**: EcoRI is a restriction enzyme that recognizes specific DNA sequences and cuts them. It is known to cut at a palindromic sequence, which means the sequence reads the same in both directions. 2. **Identifying the Recognition Sequence**: The recognition site for EcoRI is specifically known to be: - **5' - G/AATTC - 3'** - **3' - CTTAA/G - 5'** Here, the slash (/) indicates the position where EcoRI cuts the DNA, between the G and A nucleotides. 3. **Analyzing the Options**: - **(a)** `{:(G-A-Aoverset(darr)-T-T-C),(C-T-Tunderset(uarr)-A-A-G):}` - This does not match the EcoRI recognition sequence. - **(b)** `{:(G-T-Coverset(darr)-G-A-C),(C-A-Gunderset(uarr)-C-T-G):}` - This does not match the EcoRI recognition sequence. - **(c)** `{:(Goverset(darr)-T-C-G-A-C),(C-A-G-C-Tunderset(uarr)-G):}` - This does not match the EcoRI recognition sequence. - **(d)** `{:(Goverset(darr)-A-A-T-T-C),(C-T-T-A-Aunderset(uarr)-G):}` - This correctly represents the EcoRI recognition site, with the cut occurring between G and A. 4. **Conclusion**: Based on the analysis, the correct option that depicts the recognition site for EcoRI is **(d)**. ### Final Answer: **(d)** `{:(Goverset(darr)-A-A-T-T-C),(C-T-T-A-Aunderset(uarr)-G):}` ---

To determine which option correctly depicts the recognition site for EcoRI, we need to analyze the characteristics of EcoRI and its recognition site. ### Step-by-Step Solution: 1. **Understanding EcoRI**: EcoRI is a restriction enzyme that recognizes specific DNA sequences and cuts them. It is known to cut at a palindromic sequence, which means the sequence reads the same in both directions. 2. **Identifying the Recognition Sequence**: The recognition site for EcoRI is specifically known to be: - **5' - G/AATTC - 3'** ...
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