Home
Class 12
BIOLOGY
The sticky ends of a fragmented DNA mole...

The sticky ends of a fragmented DNA molecule are made of

A

(a) calcium salts

B

(b) endonuclease enzyme

C

(c) unpaired bases

D

(d) methyl groups

Text Solution

AI Generated Solution

The correct Answer is:
To answer the question, "The sticky ends of a fragmented DNA molecule are made of," we can follow these steps: ### Step-by-Step Solution: 1. **Understanding DNA Fragmentation**: - DNA can be fragmented using restriction enzymes, which cut the DNA at specific sequences. This results in either blunt ends or sticky ends. 2. **Identifying Sticky Ends**: - Sticky ends are created when the restriction enzyme cuts the DNA in such a way that it leaves overhanging sequences of unpaired bases. These overhangs are essential for the ligation process, allowing the DNA fragments to bind together. 3. **Analyzing the Options**: - A. Calcium salts: These are not related to the structure of DNA ends. - B. Endonuclease enzyme: This is the enzyme that cuts the DNA but does not form the sticky ends. - C. Unpaired bases: This option refers to the overhanging sequences that are characteristic of sticky ends. - D. Methyl groups: These are chemical modifications that can occur on DNA but do not constitute the structure of sticky ends. 4. **Conclusion**: - The sticky ends of a fragmented DNA molecule are made of **C. Unpaired bases**. These unpaired bases allow for the easy ligation of DNA fragments. ### Final Answer: C. Unpaired bases ---

To answer the question, "The sticky ends of a fragmented DNA molecule are made of," we can follow these steps: ### Step-by-Step Solution: 1. **Understanding DNA Fragmentation**: - DNA can be fragmented using restriction enzymes, which cut the DNA at specific sequences. This results in either blunt ends or sticky ends. 2. **Identifying Sticky Ends**: ...
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY - PRINCIPLES AND PROCESSES

    NCERT FINGERTIPS ENGLISH|Exercise HOTS|5 Videos
  • BIOTECHNOLOGY - PRINCIPLES AND PROCESSES

    NCERT FINGERTIPS ENGLISH|Exercise NCERT|19 Videos
  • BIODIVERSITY & CONSERVATION

    NCERT FINGERTIPS ENGLISH|Exercise Biodiversity & Conservation|140 Videos
  • BIOTECHNOLOGY AND ITS APPLICATION

    NCERT FINGERTIPS ENGLISH|Exercise Biotechnology And Its Application|137 Videos

Similar Questions

Explore conceptually related problems

In the DNA molecule

In the DNA molecule

In the DNA molecule

In a DNA molecule

A DNA fragment is

Back bone in structure of DNA molecule is made up of -

In the 5' end of a DNA molecule

Which of the following statements does not hold true for restriction enzyme? (a) It recongnises a palindromic nucleotide sequence (b) It is an endonuclease. (c) It is isolated from viruses (d) It produces the same kind of sticky ends in different DNA molecules

The ends of DNA fragments are stickly due to

DNA fragments are

NCERT FINGERTIPS ENGLISH-BIOTECHNOLOGY - PRINCIPLES AND PROCESSES-Biotechnology - Principles And Processes
  1. Study the following figures and identify the enzymes involved in steps...

    Text Solution

    |

  2. Which of the following correctly depicts the recognition site for EcoR...

    Text Solution

    |

  3. The sticky ends of a fragmented DNA molecule are made of

    Text Solution

    |

  4. The restrcition enzyme responsible for cleavage of following sequence ...

    Text Solution

    |

  5. Identify the palindromic sequence in the following

    Text Solution

    |

  6. Which of the following statements is not correct regarding EcoRl restr...

    Text Solution

    |

  7. Which of the following sequences is recongnised by restriction enzyme ...

    Text Solution

    |

  8. If a plasmid vector is digested with EcoRl at a single site, then

    Text Solution

    |

  9. How many fragments will be generated if you digest a linear DNA molecu...

    Text Solution

    |

  10. How many fragments will be generated on the digestion of a closed circ...

    Text Solution

    |

  11. The flow chart given below represents the process of recombinant DNA t...

    Text Solution

    |

  12. In recombinant DNA technology, a plasmid vector is cleaved by

    Text Solution

    |

  13. Gel electrophoresis is a

    Text Solution

    |

  14. Gel electrophoresis is used for

    Text Solution

    |

  15. Having become an expert on gel electrophoresis, you are asked to exami...

    Text Solution

    |

  16. Which of the following steps performed by person to visualise t...

    Text Solution

    |

  17. Study the given figure carefully and select the incorrect statements r...

    Text Solution

    |

  18. Which of the following tools of recombinant DNA technology is incorrec...

    Text Solution

    |

  19. If you want of recover many copies of the target DNA, you will choose ...

    Text Solution

    |

  20. Which of the following statements are correct ? (i) Restriction enzy...

    Text Solution

    |