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How many fragments will be generated if ...

How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA?
(a) 3
(b) 6
(c) 5
(d) 4

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To determine how many fragments will be generated when digesting a linear DNA molecule with a restriction enzyme that has four recognition sites, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Structure of Linear DNA**: - A linear DNA molecule has two ends and can be visualized as a straight line. 2. **Identify the Recognition Sites**: - The problem states that there are four recognition sites on the DNA molecule. These sites are where the restriction enzyme will cut the DNA. 3. **Determine the Number of Cuts**: - Each recognition site corresponds to a cut made by the restriction enzyme. Therefore, if there are four recognition sites, the enzyme will make four cuts. 4. **Calculate the Number of Fragments**: - The number of fragments generated from a linear DNA molecule can be calculated using the formula: \[ \text{Number of fragments} = \text{Number of cuts} + 1 \] - In this case, with four cuts: \[ \text{Number of fragments} = 4 + 1 = 5 \] 5. **Conclusion**: - Therefore, when a linear DNA molecule is digested with a restriction enzyme that has four recognition sites, it will produce a total of **5 fragments**. ### Final Answer: (c) 5 ---

To determine how many fragments will be generated when digesting a linear DNA molecule with a restriction enzyme that has four recognition sites, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Structure of Linear DNA**: - A linear DNA molecule has two ends and can be visualized as a straight line. 2. **Identify the Recognition Sites**: ...
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NCERT FINGERTIPS ENGLISH-BIOTECHNOLOGY - PRINCIPLES AND PROCESSES-Biotechnology - Principles And Processes
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