Match column I with column II and select the correct answer from the given codes.
`{:(Column I,Column II),(A.amp^(R) "gene",(i)"Artificial plasmid"),(B."Separation of DNA fragments",(ii)"Selectable marker"),(C."Hindlll",(iii)"Electrophoresis"),(D.pBR322,(iv)"Haemophilus influenzea"):}`

Options

A. 1) `A-(iii),B-(ii),C-(i),D-(iv)`
B. 2) `A-(iv),B-(i),C-(iii),D-(ii)`
C. 3) `A-(ii),B-(iii),C-(iv),D-(i)`
D. 4) `A-(iii),B-(iv),C-(i),D-(iii)`

Answer

To solve the matching question, we need to correlate the items in Column I with the appropriate items in Column II based on our understanding of biotechnology concepts.

### Step-by-Step Solution:

1. **Identify the first item in Column I: A. ampicillin resistance gene**
- The ampicillin resistance gene is commonly used as a **selectable marker** in genetic engineering. It helps in identifying successfully transformed cells that can survive in the presence of ampicillin.
- **Match:** A → (ii) "Selectable marker"

2. **Identify the second item in Column I: B. Separation of DNA fragments**
- The process of separating DNA fragments is typically done through **electrophoresis**, where DNA is separated based on size.
- **Match:** B → (iii) "Electrophoresis"

3. **Identify the third item in Column I: C. HindIII**
- HindIII is a restriction enzyme that is derived from the bacterium **Haemophilus influenzae**. It is used to cut DNA at specific sequences.
- **Match:** C → (iv) "Haemophilus influenzae"

4. **Identify the fourth item in Column I: D. pBR322**
- pBR322 is one of the first **artificial plasmids** used in cloning. It serves as a vector for gene cloning.
- **Match:** D → (i) "Artificial plasmid"

### Summary of Matches:
- A → (ii) "Selectable marker"
- B → (iii) "Electrophoresis"
- C → (iv) "Haemophilus influenzae"
- D → (i) "Artificial plasmid"

### Final Answer:
The correct matches are:
- A → ii
- B → iii
- C → iv
- D → i

Thus, the correct answer code is **3**.

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