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Read the given statements and select the...

Read the given statements and select the correct option.
Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes.
Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining.
1) Both statements 1 and 2 are correct
2) statement 1 is correct but statement 2 is incorrect
3) statement 1 is incorrect but statement 2 is correct
4) None of the above

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The correct Answer is:
To solve the question, we need to analyze both statements regarding cloning vectors and their recognition sites for restriction enzymes. ### Step-by-Step Solution: 1. **Understanding Statement 1**: - The statement claims that a cloning vector should have very few, preferably a single, recognition site for commonly used restriction enzymes. - This is correct because having fewer recognition sites simplifies the cloning process. If there is only one recognition site, the enzyme will cut the vector at that specific site, producing a single fragment that can be easily manipulated. 2. **Understanding Statement 2**: - This statement asserts that the presence of more than one recognition site within a cloning vector will generate several fragments, complicating the gene cloning process. - This statement is also correct. If multiple recognition sites are present, the restriction enzyme will cut at each site, resulting in multiple fragments. This can complicate the cloning process as it becomes difficult to isolate the desired fragment. 3. **Conclusion**: - Since both statements are correct, we can conclude that the correct option is: - **Option 1: Both statements 1 and 2 are correct.** ### Final Answer: The correct option is **1) Both statements 1 and 2 are correct.**
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NCERT FINGERTIPS ENGLISH-BIOTECHNOLOGY - PRINCIPLES AND PROCESSES-Biotechnology - Principles And Processes
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  2. Identify A,B,C, and D in the given figure of E. coli cloning vector pB...

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  3. Read the given statements and select the correct option. Statement 1...

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  4. pBR322 was the first artificial cloning vector to be constructed. What...

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  5. Read the following statements and select the correct ones. (i) Elect...

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  6. In pBR322, tetracycline resistance gene (tet^(R)) has recognition site...

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  7. Which of the following is not a characteristic of pBR322 vector ?

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  8. What will be the effect if pBR322, a cloning vector does not carry 'or...

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  9. Using recombinant DNA technology, genes from a donor cell can be inser...

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  10. An advantage of using yeasts rather than bacteria as recipient cells f...

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  11. Read the given statements and select the correct option. Statement 1...

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  12. In the process of insertional inactivation 1) a recombinant DNA is in...

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  13. If a person obtains transformants by inserting a recombinant DNA withi...

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  14. Read the given statements and select the correct option Statement 1 ...

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  15. During insertional inactivation, the presence of a chromogenic substra...

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  16. Which of the following bacteria is used as a vector for plant genetic ...

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  17. Which of the following microbes transform normal plant and animal cell...

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  18. Read the given statements and select the correct option. Statement 1...

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  19. a crown gall bacterium, is called an natural genetic engineer' of plan...

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  20. DNA cannot pass through a cell membrane as 1) it is too big to cross...

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