In pBR322, tetracycline resistance gene `(tet^(R))` has recognition site for which of the following restriction endonuclease ?
1) Hindlll
2) BamHl
3) EcoRl
4) Pstl

Options

A. 1) Hindlll
B. 2) BamHl
C. 3) EcoRl
D. 4) Pstl

Explanation

To determine which restriction endonuclease has a recognition site in the tetracycline resistance gene `(tet^(R))` of the plasmid pBR322, we can follow these steps:

### Step 1: Understand the structure of pBR322
pBR322 is a plasmid that contains two antibiotic resistance genes: one for tetracycline (tet^(R)) and another for ampicillin (amp^(R)).

**Hint:** Familiarize yourself with the components of pBR322, especially the antibiotic resistance genes.

### Step 2: Identify the restriction endonucleases
The question provides four options for restriction endonucleases: HindIII, BamHI, EcoRI, and PstI. We need to check which of these has a recognition site within the tetracycline resistance gene.

**Hint:** Look up the recognition sites of each of the listed restriction endonucleases.

### Step 3: Analyze the recognition sites
- **HindIII**: Recognizes the sequence AAGCTT.
- **BamHI**: Recognizes the sequence GGATCC.
- **EcoRI**: Recognizes the sequence GAATTC.
- **PstI**: Recognizes the sequence CTGCAG.

**Hint:** Compare the recognition sequences with the known sequences in the tetracycline resistance gene.

### Step 4: Match the recognition sites with the tetracycline resistance gene
From the video transcript, it is mentioned that the tetracycline resistance gene has a recognition site for BamHI. The other options, HindIII, EcoRI, and PstI, do not have recognition sites in the tetracycline resistance gene.

**Hint:** Confirm the specific restriction sites associated with the tet^(R) gene in pBR322.

### Step 5: Conclusion
Based on the analysis, the correct answer is that the tetracycline resistance gene `(tet^(R))` in pBR322 has a recognition site for **BamHI**.

**Final Answer:** 2) BamHI