What will be the effect if pBR322, a cloning vector does not carry 'ori' site ?

Options

A. 1) Sticky ends will not produce
B. 2)Transformation will not take place
C. 3) The cell will transform into a tumour cell
D. 4) Replication will not take place

Explanation

To answer the question regarding the effect if pBR322, a cloning vector, does not carry an 'ori' site, we can break down the explanation into clear steps:

### Step-by-Step Solution:

1. **Understanding the Role of 'ori' Site**:
- The 'ori' (origin of replication) site is crucial in a cloning vector as it is the specific sequence where replication begins. Without this site, the vector cannot replicate itself within a host cell.

2. **Analyzing the Statements**:
- The question presents several statements regarding the effects of the absence of the 'ori' site. We need to evaluate each statement to determine its validity.

3. **Evaluating Statement 1**:
- **Statement**: "Sticky ends will not produce."
- **Analysis**: This statement is incorrect. Sticky ends are produced by the action of restriction enzymes on the vector and are not dependent on the presence of the 'ori' site.

4. **Evaluating Statement 2**:
- **Statement**: "Transformation will not take place."
- **Analysis**: This statement is also incorrect. Transformation refers to the process of introducing foreign DNA into a host cell. As long as the genetic material is present, transformation can occur regardless of the 'ori' site.

5. **Evaluating Statement 3**:
- **Statement**: "The cell will transform into a tumor cell."
- **Analysis**: This statement is incorrect as well. The transformation of normal cells into tumor cells is related to oncogenesis, which is not influenced by the presence or absence of the 'ori' site in the vector.

6. **Evaluating Statement 4**:
- **Statement**: "Replication will not take place."
- **Analysis**: This statement is correct. Without the 'ori' site, the vector cannot initiate replication, meaning it cannot multiply within the host cell.

7. **Conclusion**:
- The absence of the 'ori' site in pBR322 will primarily result in the inability of the vector to replicate itself within the host cell. Therefore, the correct answer to the question is that replication will not take place.

### Final Answer:
If pBR322 does not carry an 'ori' site, replication will not take place.

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