Read the given statements and select the correct option
Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of `beta`-galactosidase enzyme and the colonies do not produce any colour.
A) Both statements 1 and 2 are correct
B) statement 1 is correct but statement 2 is incorrect
C) statement 1 is incorrect but statement 2 is correct
D) Both statements 1 and 2 are incorrect

Options

A. A) Both statements 1 and 2 are correct
B. B) statement 1 is correct but statement 2 is incorrect
C. C) statement 1 is incorrect but statement 2 is correct
D. D) Both statements 1 and 2 are incorrect

Answer

To solve the question, we need to analyze both statements regarding insertional inactivation and the behavior of bacterial colonies in relation to the LacZ gene and beta-galactosidase enzyme.

### Step-by-Step Solution:

1. **Understanding Insertional Inactivation**:
- Insertional inactivation occurs when a foreign DNA insert is introduced into a plasmid that contains a gene (in this case, the LacZ gene) responsible for producing an enzyme (beta-galactosidase).
- The presence of the insert disrupts the function of the LacZ gene.

2. **Analyzing Statement 1**:
- Statement 1 claims that "In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome."
- This is correct because if the plasmid does not have an insert, the LacZ gene is functional, leading to the production of beta-galactosidase, which reacts with a chromogenic substrate to produce a blue color.

3. **Analyzing Statement 2**:
- Statement 2 states that "Presence of insert results into insertional inactivation of beta-galactosidase enzyme and the colonies do not produce any colour."
- This is also correct because when the insert is present, the LacZ gene is inactivated, beta-galactosidase is not produced, and thus the colonies remain colorless.

4. **Conclusion**:
- Both statements accurately describe the process of insertional inactivation and its effects on bacterial colonies.
- Therefore, the correct option is **A) Both statements 1 and 2 are correct**.