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In biolistic method of gene trasfer, the...

In biolistic method of gene trasfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of
1) silver or tungsten
2) arsenic or silver
3) gold or tungsten
4) none of these

A

1) silver or tungsten

B

2) arsenic or silver

C

3) gold or tungsten

D

4) none of these

Text Solution

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The correct Answer is:
To solve the question regarding the materials used in the biolistic method of gene transfer, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Biolistic Method**: The biolistic method, also known as the gene gun method, involves the use of microparticles that are coated with foreign DNA. These microparticles are then accelerated and shot into target cells at high velocities. 2. **Identify the Microparticles**: The question asks about the composition of these microparticles. It's important to know what materials are typically used in this method. 3. **Evaluate the Options**: - **Option 1: Silver or Tungsten** - While tungsten is indeed used, silver is not typically used in this context. - **Option 2: Arsenic or Silver** - Neither arsenic nor silver is used in the biolistic method. - **Option 3: Gold or Tungsten** - This is a correct statement as both gold and tungsten are commonly used materials for the microparticles. - **Option 4: None of these** - This option is incorrect since option 3 is valid. 4. **Select the Correct Answer**: Based on the evaluation, the correct answer is **Option 3: Gold or Tungsten**. These materials are effective for coating with foreign DNA and are used in the biolistic method of gene transfer. ### Final Answer: The correct answer is **Option 3: Gold or Tungsten**. ---

To solve the question regarding the materials used in the biolistic method of gene transfer, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Biolistic Method**: The biolistic method, also known as the gene gun method, involves the use of microparticles that are coated with foreign DNA. These microparticles are then accelerated and shot into target cells at high velocities. 2. **Identify the Microparticles**: The question asks about the composition of these microparticles. It's important to know what materials are typically used in this method. ...
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NCERT FINGERTIPS ENGLISH-BIOTECHNOLOGY - PRINCIPLES AND PROCESSES-Biotechnology - Principles And Processes
  1. Micro-injection is a method used to

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  2. Which of the following is required for micro-injection method of gene ...

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  3. In biolistic method of gene trasfer, the microparticles coated with fo...

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  4. The different steps of recombinant DNA technology are given below rand...

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  5. The given flow chart depicts the steps to transfer a desirable gene of...

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  6. Fill up the blanks and select the correct option. (i) EcoRl cuts the...

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  7. In the isolation of DNA, removal of protein and RNA is carried out by ...

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  8. During isolation of genetic material, the chemical used to precipitate...

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  9. Precipitates of purified DNA after the addition of chilled ethanol are...

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  10. Match column I with column II and select the correct answer from the g...

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  11. The polymerase chain reaction is a technique used for 1) amplificati...

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  12. Process used for amplification or multiplication of DNA in DNA fingerp...

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  13. Primers are 1) chemically synthesised oligonucleotides that are comp...

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  14. Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium ...

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  15. Which of the following statements are correct for the enzyme Taq polym...

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  16. Match column I (enzyme) with column II (characteristic/activity) and s...

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  17. Which one of the following is not a correct match ?

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  18. The correct sequence of different steps of polymerease chain reaction ...

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  19. Given table gives an account of differences between PCR and gene cloni...

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  20. Which of the following is required to perform polymerase chain reactio...

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