The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequences of steps ?

Options

A. A) `(i) rarr (iii) rarr (ii) rarr (iv) rarr (v)`
B. B) `(iii) rarr (ii) rarr (i) rarr (v) rarr (iv)`
C. C) `(i) rarr (v) rarr (ii) rarr (iv) rarr (iii)`
D. D)`(v) rarr (i) rarr (iii) rarr (iv) rarr (ii)`

Answer

To solve the question regarding the correct sequence of steps in recombinant DNA technology, we can break down the process into a logical order based on the steps provided:

### Step-by-Step Solution:

1. **Isolation of the DNA fragments or genes to be cloned (Step i)**:
- The first step in recombinant DNA technology is to isolate the specific DNA fragment or gene that you want to clone. This involves extracting DNA from the organism and using techniques such as restriction enzymes to cut the DNA at specific sites.

2. **Insertion of the isolated gene in a suitable plasmid vector (Step v)**:
- After isolating the desired DNA fragment, the next step is to insert this fragment into a suitable plasmid vector. Plasmids are small circular DNA molecules that can replicate independently within a host cell. This step is crucial for creating recombinant DNA.

3. **Introduction of the recombinant DNA into a suitable cell (usually E. coli) called host (transformation) (Step ii)**:
- Once the recombinant DNA is constructed, it needs to be introduced into a host cell. This process is known as transformation. E. coli is commonly used as a host for cloning purposes.

4. **Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (Step iv)**:
- After transformation, it is important to select the cells that have successfully taken up the recombinant DNA. This step often involves using antibiotic resistance markers or other selection techniques to identify the transformed cells.

5. **Multiplication/expression of the introduced gene in the host (Step iii)**:
- The final step is to allow the transformed host cells to multiply and express the introduced gene. This step ensures that the desired protein or trait is produced in sufficient quantities for further study or application.

### Correct Sequence of Steps:
The correct sequence of steps in recombinant DNA technology is:
1. (i) Isolation of the DNA fragments or genes to be cloned
2. (v) Insertion of the isolated gene in a suitable plasmid vector
3. (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coli) called host (transformation)
4. (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
5. (iii) Multiplication/expression of the introduced gene in the host

### Final Answer:
The correct sequence is: **(i) → (v) → (ii) → (iv) → (iii)**