In the isolation of DNA, removal of protein and RNA is carried out by enzymes ______and_______respectively.
1) lysozyme, ribonuclease
2) protease, cellulase
3) protease, ribonuclease
4) ribonuclease, chitinase

Options

A. 1) lysozyme, ribonuclease
B. 2) protease, cellulase
C. 3) protease, ribonuclease
D. 4) ribonuclease, chitinase

Explanation

To solve the question regarding the enzymes used in the isolation of DNA for the removal of proteins and RNA, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Purpose of the Enzymes**:
- We need to find enzymes that are specifically used to remove proteins and RNA during the DNA isolation process.

2. **Enzyme for Protein Removal**:
- The enzyme that is commonly used to digest or remove proteins is **protease**. Proteases are enzymes that break down proteins into smaller peptides or amino acids.

3. **Enzyme for RNA Removal**:
- The enzyme used to remove RNA is **ribonuclease (RNase)**. Ribonuclease specifically cleaves RNA molecules, thereby removing them from the sample.

4. **Evaluate the Options**:
- Now, we will evaluate the given options:
- **Option 1**: Lysozyme and ribonuclease - Incorrect (lysozyme is not used for protein removal).
- **Option 2**: Protease and cellulase - Incorrect (cellulase is not used for RNA removal).
- **Option 3**: Protease and ribonuclease - Correct (protease removes proteins and ribonuclease removes RNA).
- **Option 4**: Ribonuclease and chitinase - Incorrect (chitinase is not used for protein removal).

5. **Conclusion**:
- The correct answer is **Option 3: protease and ribonuclease**.

### Final Answer:
The correct enzymes used for the removal of protein and RNA in the isolation of DNA are **protease** and **ribonuclease**, respectively.

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