Given table gives an account of differences between PCR and gene cloning. Which of the following points shows the incorrect difference ?
`{:("Parameter",PCR,"Gene cloning"),(1."Efficient","More","Less"),(2."Apparatus Requirement",DNA,"Restriction enzyme, ligase, vector, bacterial cell"),(3."Manipulation","in vitro","in vitro and in vivo"),(4."Cost","More","Less"),(5."Automation","Yes",No),(6."Error probability","Less","More"),(7."Time for a typical experiment","2-4days",4hours),(8."Application","More","Less"):}`
a) 1 and 3
b) 4,5 and 6
c) 4 and 7
d) 4,7 and 8

Options

A. a) 1 and 3
B. b) 4,5 and 6
C. c) 4 and 7
D. d) 4,7 and 8

Explanation

To solve the question regarding the differences between PCR (Polymerase Chain Reaction) and gene cloning, we will analyze each parameter provided in the table and identify which points show incorrect differences.

### Step-by-Step Solution:

1. **Evaluate Efficiency**:
- PCR is more efficient than gene cloning.
- **Conclusion**: This statement is correct.

2. **Apparatus Requirement**:
- PCR requires DNA, while gene cloning requires restriction enzymes, ligase, vectors, and bacterial cells.
- **Conclusion**: This statement is correct.

3. **Manipulation**:
- PCR is performed in vitro (in the lab), while gene cloning can be performed both in vitro and in vivo (inside an organism).
- **Conclusion**: This statement is correct.

4. **Cost**:
- The table states that PCR is more costly than gene cloning, which is incorrect. PCR is generally less costly due to fewer steps involved.
- **Conclusion**: This statement is incorrect.

5. **Automation**:
- PCR is automated, while gene cloning is not.
- **Conclusion**: This statement is correct.

6. **Error Probability**:
- PCR has less error probability compared to gene cloning, which has more due to human involvement.
- **Conclusion**: This statement is correct.

7. **Time for a Typical Experiment**:
- The table states that PCR takes 2-4 days and gene cloning takes 4 hours, which is incorrect. PCR typically takes around 4 hours, while gene cloning can take several days.
- **Conclusion**: This statement is incorrect.

8. **Application**:
- PCR has more applications than gene cloning.
- **Conclusion**: This statement is correct.

### Summary of Findings:
- The incorrect differences are found in points 4 (Cost) and 7 (Time).
- Therefore, the correct answer to the question is **option C (4 and 7)**.