In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction `(PCR)`?

Options

A. a) Pfu polymerase isolated from Pyrococcus furiosus
B. b) Tli polymerase( vent ploymerase) isolated fromThermococcus litoralis
C. c) Both (a) and (b)
D. d) None of these

Answer

To answer the question about other thermostable DNA polymerases used in PCR besides Taq polymerase, we can follow these steps:

### Step-by-Step Solution:

1. **Identify Taq Polymerase**:
- Taq polymerase is an enzyme derived from the bacterium Thermus aquaticus. It is known for its ability to withstand high temperatures, which is essential in PCR processes where DNA is subjected to temperatures around 94°C.

2. **Research Other Thermostable Polymerases**:
- In addition to Taq polymerase, there are other thermostable DNA polymerases that have been isolated for use in PCR. Two notable examples are:
- **Pfu Polymerase**: This enzyme is isolated from the archaeon Pyrococcus furiosus. It is known for its high fidelity in DNA synthesis.
- **Tli Polymerase**: Also known as Wnt polymerase, this enzyme is isolated from Thermococcus littoralis. It is another thermostable polymerase that is used in PCR applications.

3. **Evaluate the Options Provided**:
- The question provides four options:
a. Pfu polymerase isolated from Pyrococcus furiosus
b. Tli polymerase isolated from Thermococcus littoralis
c. Both a and b
d. None of these
- Since both Pfu and Tli polymerases are valid thermostable enzymes used in PCR, options a and b are both correct.

4. **Select the Correct Answer**:
- Since both options a and b are correct, the best choice is option c, which states that both a and b are correct.

### Final Answer:
The correct answer is **option C: Both a and b**.

---