Select the correct option to fill up balnks.
(i) ________is a natural polymer extracted from_____.
(ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______.
(iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector.
(iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA
(v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.
a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect
b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect
c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect
d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Options

A. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect
B. b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect
C. c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect
D. d) (i) size, sieving effect (ii) agarose, eas weeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Answer

To solve the question, we will fill in the blanks step by step based on the information provided in the video transcript.

### Step-by-Step Solution:

1. **First Statement:**
- The blank states: "________ is a natural polymer extracted from _____."
- From the transcript, we know that "Agarose" is the natural polymer and it is extracted from "seaweeds."
- **Filled Statement:** "Agarose is a natural polymer extracted from seaweeds."

2. **Second Statement:**
- The blank states: "The DNA fragments purified by gel electrophoresis are used in constructing ______ by joining them with ______."
- According to the transcript, the DNA fragments are used to construct "recombinant DNA" (rDNA) by joining them with a "cloning vector."
- **Filled Statement:** "The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with a cloning vector."

3. **Third Statement:**
- The blank states: "The ligation of alien DNA is carried out at a ______ present in one of the two ______ in a plasmid vector."
- The transcript indicates that the ligation occurs at a "restriction site," which is present in one of the two "antibiotic resistance genes."
- **Filled Statement:** "The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector."

4. **Fourth Statement:**
- The blank states: "________ enzyme remains active during the high temperature induced denaturation of ds DNA."
- The transcript mentions "Taq polymerase" as the enzyme that remains active during high temperatures.
- **Filled Statement:** "Taq polymerase enzyme remains active during the high temperature induced denaturation of ds DNA."

5. **Fifth Statement:**
- The blank states: "DNA fragments are resolved according to their ______ through ______ in agarose gel electrophoresis."
- From the transcript, we learn that DNA fragments are resolved according to their "size" through the "sieving effect."
- **Filled Statement:** "DNA fragments are resolved according to their size through sieving effect in agarose gel electrophoresis."

### Final Filled Statements:
- (i) Agarose is a natural polymer extracted from seaweeds.
- (ii) The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with a cloning vector.
- (iii) The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector.
- (iv) Taq polymerase enzyme remains active during the high temperature induced denaturation of ds DNA.
- (v) DNA fragments are resolved according to their size through sieving effect in agarose gel electrophoresis.

### Correct Option:
The correct option is **(a)**.